The effect of growth - mimicking continuous strain on the early stages of skeletal development in micromass culture 4

The process of skeletogenesis during embryonic development involves sor cells, all taking place in a highly dynamic environment. The overall and pressures that are hypothesized development. However, details of these mechanical cues are not well understood, ments that could address these of early skeletal development in vitro. To strain on embryonic skeletal precursor cells in micromass culture. Strain was showing a decreased number per unit area when stretch was applied at later mechanically dynamic environment can that may provide important insight into the role of mechanical loading in early skeletal development and sets the model systems. 4 in vitro Introduction During early embryonic development, the skeletal elements arise through a dynamic environment, both chemically and physically. Secreted morphogens create Moreover, an abnormal mechanical environment in utero pressure from the womb, is though to play a role in developmental dysplasia of the skeletal elements originate from the mesodermal germ layer through the process is believed to occur through passive cell movements and cellular rearrangements Various cell culture models have been developed that allow the study of early skeletal development in vitro development, it could be revealing to incorporate such cues in the micromass assay in order to fully recapitulate the in vivo process. In several studies mechanical loading has been applied to micromass cultures, however the results vary widely and the skeletal development. skeletal development in vitro Materials and Methods Cell isolation and culture then removed from the eggs, soaked in PBS, and the wing buds were removed using